A new method for primary culture of mouse dorsal root ganglion neurons [小鼠背根神经节神经细胞的原代培养新方法]

作者：Dong T.-T.; Li S.-G.^*; Tang H.-B.; Liu W.; Wang J.-L.; Yan M.-Z.; Yu J.

来源：Journal of Xi'an Jiaotong University (Medical Sciences), 2019, 40(2): 302-304.

DOI：10.7652/jdyxb201902026

• Dorsal root ganglion
• Mouse
• Neuron
• Primary culture

Objective: To establish a simple method for primary culture of mouse dorsal root ganglion neurons of high purity. Methods: The dorsal root ganglions from healthy C57BL/6 mice of 6-8 weeks were taken to obtain dorsal root ganglion neurons by using type Ⅰ collagenase and trypsin digestion. Identification and purification were evaluated by neuron specific enolase (NSE) monoclonal antibody immunocytochemistry staining. Results: The cultured primary neurons grew well and the purity could reach about 90%. The survival time was 60 days when cultured with the DMEM medium containing nerve growth factor (NGF). Conclusion: The culture program is simple and stable, and can cultivate a large number of high-purity neurons, which provides a reliable model for in-depth study of neurons. ? 2019, Editorial Board of Journal of Xi'an Jiaotong University (Medical Sciences). All right reserved.

• 单位
  三峡大学