基于分子机制的CRISPR-Cas9靶向效应预测（英文）

作者：陈钦畅; 啜国晖; 张超^*; 张清^*; 刘琦^*

来源：Science Bulletin, 2022, 67(12): 1201-1204.

• DNA
• CRISPR-Cas9
• Section
• RNA
• 分子机制

<正>The CRISPR-Cas9 system, serving as a powerful genome-editing technology, has revolutionized the life sciences. However, it exhibits off-target activities that may present severe problems in clinical applications. Although a great number of in silico models have been developed to predict CRISPR targeting efficiency and specificity, they are running into a bottleneck with the lack of a mechanistic understanding of the on-and off-target activities of Cas9.

• 单位
  同济大学; 上海交通大学