科研之友
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摘要
In order to quickly detect the genotoxicity of various pollutants in the environment, a UV-sensitive strain constructed in previous study was used to monitor the genotoxicity of various known compounds. The strain showed quick responses when incubated with the tested compound. The biosensor responded only after 0.5 h contact with mutagens and the changes in cell culture turbidity could be easily differentiated with naked eyes from the control sample. Especially, cell densities presented obvious dose-dependent characteristics. The values of limit of detection (LOD) for amphotericin B, benzyl chloride, captan and nalidixic acid were calculated as 0.32 mmol?L-1, 0.11 mmol?L-1, 0.07 μmol?L-1 and 2.14 μmol?L-1, respectively, which were much lower than that in EU standards (SOS/umu method). Using Cr (VI) as the reference compound, a potential genotoxicity estimation method was established for water samples. Three water samples were tested by this method and the obvious SOS response was observed in one sample. The toxic equivalent concentration was estimated as 0.08 μmol?L-1 Cr (VI) when the water sample was concentrated by 200 times. This provided an optional detection method in water sample genotoxicity detection.
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