科研之友
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摘要
<正>Objective:To optimize the animal model of liver injury that can properly represent the pathological characteristics of dampness-heat jaundice syndrome of traditional Chinese medicine.Methods:The liver injury in the model rat was induced byα-naphthylisothiocyanate(ANIT) and carbon tetrachloride(CCl_4) respectively,and the effects of Yinchenhao Decoction(茵陈蒿汤,YCHD),a proved effective Chinese medical formula for treating the dampness-heat jaundice syndrome in clinic,on the two liver injury models were evaluated by analyzing the serum level of alanine aminotransferase(ALT),asparate aminotransferase(AST),alkaline phosphatase(ALP), malondialchehyche(MDA),total bilirubin(T-BIL),superoxide dismutase(SOD),glutathione peroxidase(GSH-PX) as well as the ratio of liver weight to body weight.The experimental data were analyzed by principal component analytical method of pattern recognition.Results:The ratio of liver weight to body weight was significantly elevated in the ANIT and CCI_4 groups when compared with that in the normal control(P<0.01).The contents of ALT and T-BIL were significantly higher in the ANIT group than in the normal control(P<0.05,P<0.01),and the levels of AST,ALT and ALP were significantly elevated in CCI_4 group relative to those in the normal control (P<0.01).In the YCHD group,the increase in AST,ALT and ALP levels was significantly reduced(P<0.05, P<0.01),but with no significant increase in serum T-BIL.In the CCI_4 intoxicated group,the MDA content was significantly increased and SOD,GSH-PX activities decreased significantly compared with those in the normal control group,respectively(P<0.01).The increase in MDA induced by CCI_4 was significantly reduced by YCHD (P<0.05).Conclusion:YCHD showed significant effects on preventing liver injury progression induced by CCI_4, and the closest or most suitable animal model for damp-heat jaundice syndrome may be the one induced by CCI_4.
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单位中医学科学院
