科研之友
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摘要
The efficacy and quality of commercially manufactured ※Maha Varthikava watee§ preparations may differ from its authentic preparation prepared as per original recipe. Therefore, a study was carried out to investigate whether the physico-chemical properties, HPLC and UV chromatographic and spectrophotometric measurements can be used for standardization of the above drug. Purified herbals used in the original recipe of ※Maha Varthikava watee§, were finely powdered and mixed. The mixture was ground together with three juices extracted from leaves of three different species and bee＊s honey to make pills. Five commercial samples purchased from the market were compared with the authentically prepared samples. Physical properties, chemical properties and chromatographic and spectrophotometric measurements were considered as tools for standardization. For the HPLC and UV spectrophotometry, ethanol extraction was used. Data were analyzed at 0.05 significant levels. No significant differences were observed in any commercial samples with regard to fiber content, acid insoluble ash, dichloromethene extract, ethyl acetate extract and methanol extract while the weight of pills was significantly different. The pH values and loss on drying were significantly different only in two commercial samples. Specific gravity and ash values were found to be different only in one sample. Hexane extract value was significantly different in one sample. In HPLC chromatograms, one main peak was more prominent in three commercial samples. In UV spectrophotometric measurement, two commercial samples had a 竹 max of 287-290 nm while other three samples had a 竹 max of 340-345 nm. Hence, these measurements can be used for the standardization of ※Maha Varthikava watee§.
