科研之友
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摘要
Objective: To observe the in vivo effect of Danlou Tablet(丹蒌片,DLT) on myocardial ischemia and reperfusion(I/R) injury.Methods: DLT effects were evaluated in mouse heart preparation using 30-min coronary occlusion followed by 24-h reperfusion and compared among sham group(n=6),I/R group(n=8),IPC group(ischemia preconditioning,n=6) and DLT group(I/R with DLT pretreatment for 3 days,750 mg·kg-1·day-1,n=8).The effects of DLT were characterized in infarction size(IS) compared with risk region(RR) and left ventricle using the Evans blue/triphenyltetrazolium chloride double dye staining method in vivo.Furthermore,the dose-dependent effect of DLT on I/R injury was evaluated by double staining method.Five different concentrations of DLT(0.625,1.25,2.5,5 and 10 g·kg-1·day-1) were chosen in this study,and dose-response curve of DLT was obtained on these data.Results: The ratio of IS to left ventricle was significantly smaller in the DLT and IPC groups than the I/R group(P<0.05 or P<0.01),the ratio of IS to RR was also reduced in the DLT and IPC groups(P<0.01),while there were no differences in RR among the four groups(P>0.05).Experiments showed incidence of arrhythmias was reduced in the DLT group(P<0.01).Furthermore,DLT produced a dose-dependent inhibitory effect with a half maximal inhibitory concentration of 1.225 g·kg-1·day-1.Conclusions: Our research concluded that DLT was effective in reducing I/R injury in mice,and provided experimental supports for the clinical use of DLT.
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