Utilization of ancillary studies in the cytologic diagnosis of respiratory lesions: The papanicolaou society of cytopathology consensus recommendations for respiratory cytology

作者：Layfield Lester J^*; Roy Chowdhuri Sinchita; Baloch Zubair; Ehya Hormoz; Geisinger Kim; Hsiao Susan J; Lin Oscar; Lindeman Neal I; Roh Michael; Schmitt Fernando; Sidiropoulos Nikoletta; VanderLaan Paul A

来源：Diagnostic Cytopathology, 2016, 44(12): 1000-1009.

DOI：10.1002/dc.23549

• immunocytochemistry
• flow cytometry
• molecular diagnostics
• next-generation sequencing
• cytology

The Papanicolaou Society of Cytopathology has developed a set of guidelines for respiratory cytology including indications for sputum examination, bronchial washings and brushings, CT-guided FNA and endobronchial ultrasound guided fine needle aspiration (EBUS-FNA), as well as recommendations for classification and criteria, ancillary testing and post-cytologic diagnosis management and follow-up. All recommendation documents are based on the expertise of committee members, an extensive literature review, and feedback from presentations at national and international conferences. The guideline documents selectively present the results of these discussions. The present document summarizes recommendations for ancillary testing of cytologic samples. Ancillary testing including microbiologic, immunocytochemical, flow cytometric, and molecular testing, including next-generation sequencing are discussed. Diagn. Cytopathol. 2016;44:1000-1009.