科研之友
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摘要
Nicotine is the more abundant component in cigarette smoke. Because nicotine is first metabolized in the liver, our aim was to invest赤gate the effects of nicotine on this organ by biochemical and stereological methods. Male Wistar rats were treated with oral nicotine (ON) diluted in drinking water during 32 days. The control group was treated with drinking water in the same period. Rats were sacrificed 24 hours after last day, the blood was collected and the liver was removed. Lipidogram was performed by enzymatic method and collagen fibers, fat globules and hepatocytes were count in the liver by stereological methods. We observed in control group preserved hepatocytes, with no presence of inflammatory cells. However in the ON group was possible to observe varied size of hepatocytes with cloudy cellular limits and histoarchitecture loss. Capillaries were fully of red blood cells. We observed also an increase of fat globules with small size. We observed in leucogram a reduction of leukocytes number (lymphocytes, neutrophils and monocytes) in the ON group in comparison to control group. The lipidogram showed an increase of triglycerides and total cholesterol for ON group when compared to control group. Moreover, a reduction of HDL- cholesterol was observed in ON group when compared to control group. Numerical density of hepatocytes was lesser in ON group when compared to control group. We suggest harmful effects of oral nicotine in liver induced by toxic mechanism with reduction of hepatocytes number and disturbance in lipid metabolism. La nicotina es el componente m芍s abundante en el humo del cigarrillo. Primero porque la nicotina es metabolizada en el h赤gado, nuestro objetivo fue investigar los efectos de la nicotina sobre este 車rgano por m谷todos bioqu赤micos y estereol車gicos. Hombre rata Wistar fueron tratados con la nicotina oral (NO) diluida en el agua potable durante 32 d赤as. El grupo control fue tratado con agua potable en el mismo per赤odo. Las ratas se sacrificaron 24 horas despu谷s del 迆ltimo d赤a, se recogi車 la sangre y el h赤gado fue retirado. Lipidogram se realiz車 por el m谷todo enzim芍tico y fibras de col芍geno, grasa y hepatocitos se cuentan en el h赤gado mediante m谷todos estereol車gicos. Hemos observado en el grupo control hepatocitos conservados, sin presencia de c谷lulas inflamatorias. Sin embargo en el grupo ON fue posible observar variado tama o de hepatocitos con nubes y claros l赤mites celulares y histoarchitecture p谷rdida. Capilares est芍n plenamente de los gl車bulos rojos. Se observ車 tambi谷n un aumento de grasa con peque o tama o. Hemos observad
