科研之友
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摘要
Objective To observe the effect of ecdysterone(EDS)on the level of VEGF protein in the brain,angiogenesis and neurologic function after focal cerebral ischemia in rats.Methods Rat with focal cerebral ischemia were established by occluding their middle cerebral artery.The established rats(n=36)were randomly and equally divided into EDS treatment group and ischemia group.EDS(20 mg·kg-1·d-1 for 7 d)was intraperitoneally injected into the rats of EDS treatment group 2 h after operation,and the animal of ischemia group received an intraperitoneal injection of the same solvent as in EDS group.Another 6 rats served as normal control.Rats were sacrificed in 7,14 and 21 d after operation,and the VEGF protein level and microvessel density(MVD)was detected with immunohistochemical methods and analyzed quantitatively with image system.Effect of EDS on neurologic recovery following brain ischemia were assessed using the neurologic severity scores(NSS).Results VEGF expression was not seen in normal control,and was higher in ischemia group than in the EDS treatment group at day 7 and 14,but the significant difference was only observed at day 7(P<0.01).VEGF expression was disappeared at day 21 in ischemia group.EDS treatment group had signi-ficantly higher MVD than in the ischemia group at all time points.NSS results indicated middle cerebral artery occlusion led significant neurologic impairment and subsequently certain degrees of autogenous recovery were observed.Recovery of these indices was significantly better in the EDS treatment group than in ischemia group at day 7(P<0.05)and 14(P<0.01).Conclusion EDS can definitely induce VEGF expression and angiogenesis in areas around ischemic brain regions in rats with focal cerebral ischemia,and promote neurologic recovery following the injury.
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单位第三军医大学西南医院; 神经内科
