科研之友
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摘要
Objective The purpose was to analyze the chemical compositions and their anti-inflammatory activities from Elsholtzia bodinieri. Methods The separation and purification of compounds were performed by normal-phase silica gel chromatography column (CC), Sephadex LH-20 CC, ODS CC, and high-performance liquid chromatography (HPLC). The structures of the isolated compounds were identified based on the nuclear magnetic resonance spectroscopies (NMR) data and comparison with corresponding data of the reported literature. All the isolated compounds were assessed for their anti-inflammatory effects via detecting inflammatory mediator releases (IL-6, IL-1β, TNF-α) and NO medium level in RAW 264.7 macrophage cells induced by LPS. Results A total of 19 compounds were isolated from the ethyl acetate layer of E. bodinieri, including 7 flavonoids, 5 sesquiterpenoids, 3 triterpenoids, 3 steroids and 1 phenylpropanoids compound. The structures of these isolates were unambiguously identified as apigenin (1), luteolin (2), chrysin (3), luteolin-7-O-β-D-glucoside (4), eriodictyol (5), pinocembrin (6), semecarpanone (7), caffeic acid ethylene ester (8), oplodiol (9), lβ,4β,6β-trihydroxyeudesmane (10), 1β,10α,4β,5α-diepoxy-7αH-germacran-6β-ol (11), teucladiol (12), caryolane-1,9β-diol (13), oleanelic acid (14), ursolic acid (15), pomolic acid (16), 5α,8α-epidioxy-24(S)-methylergosta-6, 9(11), 22-trien-3β-ol (17), β-sitoster (18), stigmasterol (19). Conclusion Compounds 7, 9—13 and 17 are isolated from this genus Elsholtzia for the first time. Compounds 1, 4, 9, 12—14 and 17—19 significantly reduce the release of NO and inhibited the secretion of inflammatory mediators (IL-6、IL-1β、TNF-α) in LPS-stimulated RAW 264.7 macrophages cells at the concentration of 20 μg·mL?1, comparable to DXM used as the positive control, which suggested that E. bodinieri might be possess the anti-inflammatory activity. ? 2023 Editorial Office of Chinese Traditional and Herbal Drugs.
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