Role of dietary tea polyphenols on growth performance and gut health benefits in juvenile hybrid sturgeon (Acipenser baerii ♀ x A. schrenckii ♂)

作者：Zhao, Zhenxin^*; Zhao, Fei; Cairang, Zhuoma; Zhou, Zhou; Du, Qiang; Wang, Jinle; Zhao, Feng; Wang, Qifu; Li, Zhengyou; Zhang, Xiaoping^*

来源：Fish & Shellfish Immunology, 2023, 139: 108911.

DOI：10.1016/j.fsi.2023.108911

• Hybrid sturgeon
• Tea polyphenols
• Growth performance
• Non-specific immunity
• Intestinal microbiota

The present study aimed to evaluate the effects of dietary TPs on growth performance, intestinal digestion, microflora and immunity in juvenile hybrid sturgeon. A total of 450 fish (97.20 & PLUSMN; 0.18 g) were randomly divided into a standard diet (TP-0) or four treatments consisting of a standard diet supplemented with four concentra-tions of TPs (mg/kg): 100 (TP-100), 300 (TP-300), 500 (TP-500), and 1000 (TP-1000) for 56 days. The TP-300 significantly increased weight gain rate (WGR) and specific growth rate (SGR) (p G 0.05), and TP-1000 signif-icantly increased the feed conversion ratio (FCR) (p G 0.05). TP-300 and TP-500 significantly increased intestinal trypsin, amylase, and lipase activities (p G 0.05). Besides, TP-300 significantly enhanced total antioxidant ca-pacity (T-AOC) and the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) and decreased malondialdehyde (MDA) content (p G 0.05). Moreover, TP-300 decreased the expression levels of tumor necrosis factor-alpha (TNF-& alpha;), interleukin 8 (IL-8), and interleukin 1 & beta;(IL-1 & beta;) compared with TP-0 and TP-1000 (p G 0.05). In addition, the intestinal microbiota diversity in the TP-300 group was observably higher, the dominant microbiota was Bacteroidota, Cyanobacteria, Proteobacteria and Firmicutes at the phylum level, Enterobacteriaceae, Nostocaceae and Clostridiaceae at the family level. The relative abundances of potential pro-biotics including Rhodobacteraceae and potential pathogens especially Clostridiaceae were the highest, and lowest, respectively. In conclusion, TP-300 altered the abundance of microbial taxa, resulting in enhancing the intestinal digestion, antioxidant status and non-specific immunity to improve the growth performance in juvenile hybrid sturgeon.

• 单位
  贵州省农业科学院