Zhenxin Anshen formula(镇心安神方)ameliorates atopic der-matitis-like skin dysfunction in mice and in vitro via regulation of transient receptor potential vanilloid 1 and transient receptor potential ankyrin 1 in Neural pathways

作者：ZHAO Yiding; YAN Xiaoning; JIANG Shanshan; LIU Yong; DONG Chun; CHI Huiyan^*; MAO Chaoyi^*

来源：Journal of Traditional Chinese Medicine, 2023, 43(05): 887-896.

DOI：10.19852/j.cnki.jtcm.20230802.003

OBJECTIVE: To investigate the efficacy of Zhenxin Anshen formula(镇心安神方, ZXAS) on atopic dermatitis(AD) by transient receptor potential vanilloid 1(TRPV1) and transient receptor potential ankyrin 1(TRPA1) signalling pathway in mice and in vitro. METHODS: AD-like lesions were induced by 1-chloro-2,4-dinitrobenzene(DNCB) to the shaved dorsal skin of BALB/c mice. BALB/c mice were divided into five groups: normal control, model control, cetirizine, low-, medium-, and high-dose of ZXAS. After ZXAS in-tervention, the skin lesions and blood samples were collected for hematoxylin and eosin-stained and measuring the concentrations of inflammatory cytokines. Immunoglobulin E(IgE), interleukin(IL)-4, IL-5, IL-13, and thymic stromal lymphopoietin(TSLP) were de-tected by Enzyme-linked immunosorbent assay(ELISA). The spinal cords were collected for measuring the expression of gastrin-releasing peptide receptor(GRPR), TRPV1, and TRPA1 by using immunohistochemistry, western blotting, and quantitative real-time polymerase chain reaction(qR T-PCR) analyses. In addition, 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide(MTT) assay, flow cytometry, ELISA, and Western blotting were conducted for analysis of primary dorsal root ganglia(DRG) neurons in vitro. RESULTS: ZXAS treatment improved DNCB-induced AD-like lesions through reducing dermatitis score, number of scratching and epidermal thickness, accompanied by the de-creased IgE and Th2 inflammatory cytokines. ZXAS also supressed the mRNA and protein expression of GRPR, TRPV1, and TRPA1 in the spinal cord. The medicated sera of ZXAS decreased capsaicin-induced Ca2+ influx and downregulated the expression of TRPV1, TRPA1, and phospholipase C in DRG neurons. CONCLUSIONS: The therapeutic effect of ZXAS on AD may be related to the regulation of TRPV1 and TRPA1 and inhibition of Ca2+ signals in neurons.

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  中医学科学院