科研之友
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摘要
reproductive performance of sheep varies between different races. among the factors that affect the reproductive capacity, the level of expression of estrogen and progesterone receptors in the genital tract appears to have a relevant role. in the endometrium, oviduct and ovarian estrogen and progesterone regulate the expression of numerous proteins involved in morphofunctional development. genetic factors such as race would be related to expression levels of these receptors. the texel ewes have high fertility rates, are very prolific and have a high percentage of multiple births which could be related to the expression of these receptors in the reproductive tract. the aim of this study was to evaluate the expression of estrogen and progesterone receptors in the genital tract of ewes and texel high prolificacy. the expression of the receptor protein of both receptors was detected by immunohistochemistry and the level of expression of the transcripts by quantitative rt-pcr real-time. the results showed immunohistochemical expression of estrogen receptor mainly in areas of glandular and caruncular endometrium. it also highlights a lower expression of both receptors in ovarian tissue, epithelium of the oviduct and cervix. expression of progesterone receptor immunohistochemical level is much lower with weaker signal in endometrium and ovaries. the expression level of transcripts remains the same distribution as immunohistochemical signals for both receptors. we conclude that both receptors are expressed in the reproductive system texel ewes in a distribution similar to that found in other breeds of sheep, must be defined if the levels of expression are similar in different races.
