摘要

<正>分化培养液:高糖Dulbecco's modified Eagle'smedium(Gibco 12430);15%胎牛血清(BiochromS0615);0.1 mmol/L非必需氨基酸(Gibco 11140-050);2 mmol/L谷氨酰胺(Gibco 25030);0.1 mmol/Lβ-巯基乙醇(Gibco 21985)。胚胎干细胞(embryonic stem cells)在一定的培养