Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition Atrofia de la Pared Intestinal y Aumento de la Secreci車n de Sulfomucinas en el Epit谷lio Yeyunal de Ratas Sometidas a Intensa Desnutrici車n Prot谷ica

作者：Cassia Luiza de Marins Franco; Debora de Mello Gonales Sant' Ana; Eduardo Jose de Almeida Araujo

来源：International Journal of Morphology, 2010.

• Desnutricion proteico-energetica
• Intestino delgado
• Morfometria
• Mucinas
• Protein-energetic malnutrition
• Small intestine
• Morphometry
• Mucins

The effects of severe protein malnutrition on the morphometry of the intestinal wall in the jejunum of adult rats were evaluated in this study. Six 90-day-old Wistar rats (Rattus norvegicus) were assigned into two groups: CG (Control Group, n = 3) and MG (Malnourished Group, n = 3). CG animals were fed a 26%-protein chow whereas controls were fed a 4%-protein chow. After 90 days, part of the jejunum was collected and subjected to routine histology. HE-staining and histochemical techniques - Periodic Acid Schiff (PAS) and Alcian Blue (AB), pH 2.5/pH 1.0 - were used for the detection of glycoconjugates in 4米m -thick transverse cuts. Morphometric analysis of the HE-stained cuts revealed a decrease of the total thickness of the jejunal wall, mostly on the following layers: external muscle and mucosa - enterocyte height also decreased. Cuts stained by using histochemical techniques for the detection of glycoconjugates revealed maintenance of goblet cells reactive to PAS and AB pH 2.5, whereas the number of cells reactive to AB pH 1.0 increased. Protein malnutrition was concluded to provoke severe atrophy of the jejunal wall and an increase of sulphomucin secretion within the intestinal epithelium. Fueron evaluados los efectos de la desnutrici車n proteica severa sobre la morfometr赤a de la pared intestinal del yeyuno de ratas adultas . Para esto, se utilizaron seis ratas (Rattus norvegicus) Wistar, con 90 d赤as de edad, distribuidas en dos grupos: GC (grupo control, n=3) y el GD (grupo desnutrido, n=3). Los animales del GC recibieron raci車n con un contenido proteico de 26% y los del GD raci車n con 4% de prote赤nas. Despu谷s de 90 d赤as de experimentaci車n, parte del yeyuno fue retirado y sometido a un procesamiento histol車gico de rutina. Cortes transversales de 4 米m fueron te idos con HE y t谷cnicas histoqu赤micas (Periodic Acid Schifff (PAS) y Alcian Blue (AB) pH 2,5 y pH 1,0.) para evidenciar glicoconjugados. El an芍lisis morfom谷trico te idos con HE demostr車 reducci車n en el grosor total de la pared del yeyuno, especialmente de las t迆nicas muscular y mucosa, adem芍s se observ車 una disminuci車n en la altura de los entericitos. Los cortes te idos con t谷cnicas histoqu赤micas revelaron que el n迆mero de c谷lulas caliciformes reactivas al PAS y al AB pH 2,5 se mantuvo; por otro lado, hubo un aumento en el n迆mero de c谷lulas reactivas al AB pH 1,0. Se concluye que la desnutrici車n proteica severa provoca atrofia de la pared yeyunal y aumento de la secreci車n de sulfomucinas en el epitelio intestinal.