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摘要
AIM: Caffeine metabolite ratios in urine can be used to evaluate the activity of CYP1A2, CYP2A6, NAT2 and XO in vivo. In this study, an HPLC method was developed to quantify the concentrations of caffeine metabolites in 24 Chinese healthy volunteers. METHODS: 100 mg of caffeine was given to each subject as a probe drug and the urine samples of 0-12 h were collected. Urine samples were extracted with chloroform/isopropanol (9∶1, v/v) under acerbic environment (pH 3.5) and separated on a Hypersil BDS C18 column with gradient elution. The mobile phase was composed of phase A (acetonitrile) and phase B (10 mmol/L ammonium formate/formic acid (998/2, v/v) (pH 3.5) from 98/2 to 70/30, the detection wave length was 280 nm. CYP1A2 phenotype was calculated from the metabolite ratio of (AFMU+1X+1U)/17U, CYP2A6 from the ratio of 17U/(17U+17X+1U+1X+AFMU), NAT2 from the ratio of AFMU/(AFMU+1U+1X) and XO from the ratio of 1U/1X+1U. RESULTS: The calibration curves of AFMU, 1U, 1X, 17U, 17X, 137X were linear over the range o...
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