Murine brain endothelial cells differently modulate interferon-污 and interleukin-17 production in vitro

作者：Momilovi Miljana; Miljkovi D; Mostarica Stojkovi Marija

来源：Archives of Biological Sciences, 2009, 61(1): 29-36.

DOI：10.2298/abs0901029m

• CNS
• cytokine
• inflammation
• bEnd.3
• lymph node
• concanavalin A

Brain endothelial cells (BEC) are the major constituents of the blood-brain barrier (BBB), the structure that controls entrance of immune cells into CNS parenchyma. Our aim was to investigate the influence of BEC on production of IL-17 and IFN-污-cytokines that are important for CNS inflammation. To that end, co-cultivations of the bEnd.3 brain endothelial cell line and lymph node cells (LNC) were performed, and gene expression and production of IL-17 and IFN-污 were determined. It was found that bEnd.3 cells inhibited expression and production of IFN-污, but not of IL-17. Additionally, bEnd.3 cells also reduced production of the major IFN-污-promoting cytokine - IL-12 - in LNC. The observed variation in modulation of pro-inflammatory cytokines by BEC could be of importance for the understanding of CNS inflammation.