科研之友
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摘要
purpose: observe the relation between small intestine%26apos;s mucosal injury and bacterial translocation. methods: 50 adult female rats were distributed in 5 groups: 1. control: intraperitoneal injection of pseudomonas aeruginosa, 2. drainage: intraperitoneal injection of p. aeruginosa and drainage of the abdominal cavity, after 6 hours, 3. washed: intraperitoneal injection of p. aeruginosa and washing of the abdominal cavity, after 6 hours, 4. washed + drainage: intraperitoneal injection of p. aeruginosa plus drainage and washing of the cavity, after 6 hours, 5. normal: evaluation of the normal intestinal wall. after death, blood and peritoneal cultures were performed. fragments of jejunum were processed histologically for morphometric measure of the total thickness of the jejujum%26apos;s wall in comparison to the thickness of the mucosa + submucosa layer. results: in blood culture, there was growth of pseudomonas aeruginosa and escherichia coli in 90% and 52,5% of the animals, respectively. in the peritoneal culture, there was growth of p aeruginosa, e.coli and klebsiella sp in 87,5%, 85% and 5% of the animals. about the histological analysis and the thickness of the mucosa + submucosa%26apos;s layer, there was not significant alteration. conclusion: acute sepsis did not develop any thickness alteration in the small intestine%26apos;s mucosa layer. bacterial translocation can not be a direct consequence produced by mucosal intestinal injury.
