Avaliaˋˋo das metodologias M.I.C.E.ˋ, Etestˋ e microdiluiˋˋo em caldo para determinaˋˋo da CIM em isolados cl赤nicos

作者：Campana; Eloiza Helena; Carvalhaes; Cecilia Godoy; Barbosa; Paula Peraro; Machado; Antonia Maria de Oliveira; Paula; Ana Maria de; Gales; Ana Cristina

来源：Jornal Brasileiro de Patologia e Medicina Laboratorial, 2011.

DOI：10.1590/S1676-24442011000200011

• minimal inhibitory concentration
• antimicrobial
• antimicrobial susceptibility testing
• etest
• m.i.c.e.

introduction: the oxoidˋ m.i.c.evaluatortm methodology (m.i.c.e., thermo fisher scientific, basingstoke, uk), recently released into the market, represents a rapid alternative to antimicrobial susceptibility testing. objective: the objective of this study was to evaluate the performance of m.i.c.e. methodology in relation to broth microdilution (reference test) and etestˋ (biom谷rieux, marcy l%26apos;谷toile, france). material and method: a total of 160 bacterial isolates were collected comprising the following species: p. aeruginosa (20), acinetobacter spp. (20), k. pneumoniae (20), e. coli (20), s. aureus (20), coagulase-negative staphylococcus (20), e. faecalis (20) and e. faecium (20). following clinical laboratory standands institute (clsi) standards (2009) and the manufacturers%26apos; recommendations, antimicrobial susceptibility testing was performed using broth microdilution method, etest and m.i.c.e. the results were interpreted according to the criteria established by clsi and compared through regression analysis. results: all antimicrobial combinations vs. bacterial species were evaluated and m.i.c.e. methodology yielded good results with general correlation (mic variation ㊣ 1-log2) %26gt; 90%, except for cefotaxime (85%) and vancomycin (76.3%) when compared with the reference method. the m.i.c.e. results compared to etest showed general correlation (%26gt; 96%), except for amoxicillin/clavulanic acid (67.5%) combination. conclusion: ast results obtained from m.i.c.e. methodology showed a good correlation with those from broth microdilution and etest, which corroborates its time effectiveness in the determination of mic. however, the combination of amoxicillin/clavulanic acid requires further attention.