Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

作者：Shin Hyun Yong; Lee Jin Young; Park Chulhwan; Kim Seung Wook^*

来源：Journal of Biotechnology, 2011, 151(4): 363-368.

DOI：10.1016/j.jbiotec.2010.12.021

• Cysteine
• Acremonium chrysogenum
• Methionine
• Cephalosporin C

In this study, the role of glycerol in cephalosporin C production was evaluated in Acremonium chrysogenum M35. Methionine-unsupplemented culture with glycerol stimulated cephalosporin C production in A. chrysogenum M35 by up to 10-fold while increasing the amount of cysteine. Glycerol caused the upregulation of isopenicillin N-CoA epimerase (cefD2) and delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthase (pcbAB) transcription in exponential phase. These results indicate that glycerol without methionine effectively enhanced cephalosporin C production via stimulation of cysteine and valine in A. chrysogemun M35.