科研之友
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AIM：The mechanism of exosomes derived from embryonic stem cells on hypoxic cardiomyocytes is still unknown. This study explores the role of exosomal microRNAs（miRNAs）in embryonic stem cells. METHODS：Exosomes secreted by embryonic stem cell line H9 were isolated and identified. The AC16 cardiomyocytes were treated with exosomes under hypoxic conditions. The effects of exosomes on hypoxic cardiomyocytes were analyzed by Western blot，cell viability experiment，flow cytometry and mitochondrial membrane potential detection. Moreover，transcriptome sequencing of H9 cell-derived exosomes was performed to analyze the possible mechanism by which high-abundance miRNAs protected hypoxic cardiomyocytes. RESULTS：The H9 cell-derived exosomes were successfully isolated and proved to promote the survival of AC16 cells under hypoxia. The H9 cell-derived exosomes rescued the reduction of mitochondrial membrane potential，and reduced cell apoptosis. After transcriptome sequencing，we found miR-302a/b/d-3p was abundantly expressed in exosomes. Moreover，compared with control group，miR-302a/b/d-3p in AC16 cells was also significantly increased after treatment with exosomes. CONCLUSION：The H9 cell-derived exosomes reduce the apoptosis of AC16 cells under hypoxia，and miR-302a/b/d-3p in exosomes may serve as a critical role. ? 2022 Editorial Board of Chinese Journal of Pathophysiology, Jinan University.
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