Optogenetic Control of Voltage‐Gated Calcium Channels

作者:Dr. Guolin Ma; Jindou Liu; Yuepeng Ke; Xin Liu; Prof. Minyong Li; Prof. Fen Wang; Prof. Gang Han; Prof. Yun Huang; Prof. Youjun Wang; Prof. Yubin Zhou
来源:Angewandte Chemie, 2018, 130(24): 7137-7140.
DOI:10.1002/ange.201713080

摘要

Voltage‐gated Ca\u003csup\u003e2+\u003c/sup\u003e (Ca\u003csub\u003eV\u003c/sub\u003e) channels mediate Ca\u003csup\u003e2+\u003c/sup\u003e entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca\u003csub\u003eV\u003c/sub\u003e channel modulators, to enable photo‐tunable modulation of Ca\u003csub\u003eV\u003c/sub\u003e channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for Ca\u003csub\u003eV\u003c/sub\u003e channels could find broad applications in interrogating a wide range of Ca\u003csub\u003eV\u003c/sub\u003e‐mediated physiological processes.

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