Optogenetic Control of Voltage‐Gated Calcium Channels

作者：Dr. Guolin Ma; Jindou Liu; Yuepeng Ke; Xin Liu; Prof. Minyong Li; Prof. Fen Wang; Prof. Gang Han; Prof. Yun Huang; Prof. Youjun Wang; Prof. Yubin Zhou

来源：Angewandte Chemie, 2018, 130(24): 7137-7140.

DOI：10.1002/ange.201713080

• Calcium-Signalwege
• Ionenkan?le
• Optogenetik
• Protein-Engineering
• Synthetische Biologie

Voltage‐gated Ca\u003csup\u003e2+\u003c/sup\u003e (Ca\u003csub\u003eV\u003c/sub\u003e) channels mediate Ca\u003csup\u003e2+\u003c/sup\u003e entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca\u003csub\u003eV\u003c/sub\u003e channel modulators, to enable photo‐tunable modulation of Ca\u003csub\u003eV\u003c/sub\u003e channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for Ca\u003csub\u003eV\u003c/sub\u003e channels could find broad applications in interrogating a wide range of Ca\u003csub\u003eV\u003c/sub\u003e‐mediated physiological processes.

• 单位
  北京师范大学