摘要
Voltage‐gated Ca\u003csup\u003e2+\u003c/sup\u003e (Ca\u003csub\u003eV\u003c/sub\u003e) channels mediate Ca\u003csup\u003e2+\u003c/sup\u003e entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca\u003csub\u003eV\u003c/sub\u003e channel modulators, to enable photo‐tunable modulation of Ca\u003csub\u003eV\u003c/sub\u003e channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for Ca\u003csub\u003eV\u003c/sub\u003e channels could find broad applications in interrogating a wide range of Ca\u003csub\u003eV\u003c/sub\u003e‐mediated physiological processes.
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