Measurement of binding forces between host cucurbit［7］uril and guest molecules by single-molecule force spectroscopy

作者：Li, Hao; Zhang, Mingyang; Qian, Gongming; Li, Yunchuan; Guo, Jing^*

来源：Chinese Journal of Analysis Laboratory, 2023, 42(3): 384-391.

DOI：10.13595/j.cnki.issn1000-0720.2021.121301

• Benzimidazoles
• Benzothiazoles
• Binding forces
• Cucurbit[7]uril
• Guest molecules
• Host guest interactions
• Interaction forces
• Measurements of
• Protonated
• Single molecule force spectroscopy

The interactions between host cucurbit［7］uril （CB［7］）and three guests with benzothiazole，benzimidazole and benzoxazole groups in macrocyclic host molecules were studied by single-molecule force spectroscopy（SMFS），and the interaction forces of three host-guest systems were measured and compared under different loading rates. The changes of interaction forces between three kinds of guest molecules and cucurbit［7］uril under different pH were studied. The results showed that the interaction forces between protonated guest molecules and cucurbit［7］uril were less than that between non-protonated guest molecules and cucurbit［7］uril. Based on the analysis of nuclear magnetic mass spectrometry and the data of single-molecule force spectra，it was speculated that hydrophobic interaction played an important role in the binding force. The results were helpful for the rational design and construction of possible supramolecules as intelligent nanocarriers for anticancer drug delivery.

• 单位
  武汉科技大学