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摘要
Phanerochaete chrysosporium, one typical species of white rot fungi, was chosen as the experimental strains to investigate the effect of environmental condition on TPhP biodegradation by P. chrysosporium and the changes of cellular characteristics of P. chrysosporium during TPhP biodegradation. The results showed that the biodegradation efficiency of 5mg/L TPhP by P. chrysosporium was above 60% when inoculum size was 4% (V/V), pH was adjusted to 5~6 and initial glucose concentration was 5g/L. Cytochrome P450 of P. chrysosporium played an important role in TPhP biodegradation, the inhibition of cytochrome P450 could result in the decrease of TPhP biodegradation efficiency. In the earlier stage of TPhP biodegradation, the intracellular protein content and ATPase activity of P. chrysosporium increased remarkably to promote the metabolism and biotransformation of TPhP. Under the stress of TPhP, SOD and CAT activities of P. chrysosporium increased firstly and then decreased. There was a synergistic action between these two antioxidant enzymes in maintaining the intracellular redox balance during TPhP biodegradation. ? 2020, Editorial Board of China Environmental Science. All right reserved.
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