Bph6 encodes an exocyst- localized protein and confers broad resistance to planthoppers in rice

作者：Guo, Jianping; Xu, Chunxue; Wu, Di; Zhao, Yan; Qiu, Yongfu; Wang, Xiaoxiao; Ouyang, Yidan; Cai, Baodong; Liu, Xin; Jing, Shengli; Shangguan, Xinxin; Wang, Huiying; Ma, Yinhua; Hu, Liang; Wu, Yan; Shi, Shaojie; Wang, Wenliang; Zhu, Lili; Xu, Xun; Chen, Rongzhi; Feng, Yuqi; Du, Bo^*; He, Guangcun^*

来源：Nature Genetics, 2018, 50(2): 297-+.

DOI：10.1038/s41588-018-0039-6

• MAP-BASED CLONING
• BROWN PLANTHOPPER
• GENE-EXPRESSION
• SALICYLIC-ACID
• NILAPARVATA-LUGENS
• PLANT
• DEFENSE
• IDENTIFICATION
• PHYTOALEXINS
• CYTOKININS

The brown planthopper (BPH) and white-backed planthopper (WBPH) are the most destructive insect pests of rice, and they pose serious threats to rice production throughout Asia. Thus, there are urgent needs to identify resistance-conferring genes and to breed planthopper-resistant rice varieties. Here we report the map-based cloning and functional analysis of Bph6, a gene that confers resistance to planthoppers in rice. Bph6 encodes a previously uncharacterized protein that localizes to exocysts and interacts with the exocyst subunit OsEXO70E1. Bph6 expression increases exocytosis and participates in cell wall maintenance and reinforcement. A coordinated cytokinin, salicylic acid and jasmonic acid signaling pathway is activated in Bph6-carrying plants, which display broad resistance to all tested BPH biotypes and to WBPH without sacrificing yield, as these plants were found to maintain a high level of performance in a field that was heavily infested with BPH. Our results suggest that a superior resistance gene that evolved long ago in a region where planthoppers are found year round could be very valuable for controlling agricultural insect pests.

• 单位
  华中农业大学; 深圳华大生命科学研究院; 武汉大学; 杂交水稻国家重点实验室