摘要
:The vitamin C content and total phenolic content of five juices were compared. The phenolic profiles of juices were determined by high performance liquid chromotography, and the antioxidant and antiproliferative activities towards tumor cells were evaluated by chemical and cellular assays. The results showed that Rosa roxburghii juice exhibited the highest vitamin C content (14.13 g/L). The total polyphenol content of R. roxburghii and sea buckthorn juices was 13.06 and 12.53 g GAE/L, respectively, which was at the highest level and no significant difference was observed between them (P>0.05). Four phenolic compounds, gallic acid, rutin, catechin and ferullic acid were identified from five juices. The antioxidant activity of juices showed significantly correlations with the content of both gallic acid and total phenolic. Sea buckthorn, R. roxburghii and blueberry juices exhibited high PSC and ORAC activities, while lemon and mandarin juices showed low activities and no significant difference was observed between them (P>0.05). R. roxburghii juice (163.96 μmol QE/100 mL) and sea buckthorn juice (82.68 μmol QE/100 mL) exhibited the highest CAA activity with and without PBS wash, respectively, and sea buckthorn juice had the highest cell absorptivity (65.27%). R. roxburghii juice showed the highest antiproliferative activity towards tumor cells (EC50=5.50 mg/mL) and the lowest cytotoxicity (CC50>200 mg/mL). Five juices were divided into two categories in terms of the phenolic profiles and the antioxidant activity. One was R. roxburghii and sea buckthorn juices, and the other was lemon, blueberry and mandarine juices. This study could provide the theoretical guidance for the development of fruit deep processing industry and functional drink. ? 2022, Editorial Department of Science and Technology of Food Science.
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