科研之友
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摘要
In order to explore the change rule of myoblast stem cells (satellite cells, SCs) in the denervated and re-innervated muscle and to investigate the cellular mechanism of the morphological and functional changes of the muscle, denervated muscle atrophy and nerve regeneration models were established in one-month-old rats. Postoperative indexes such as muscle wet weight, cell section areas, content of collagen fibers and DNA, electrophysiology, numbers of SCs in the triceps muscle of calf were dynamically tested. After denervation, the muscle wet weight and cell area reduced rapidly, and the collagen fiber content increased slowly. The number of SCs increased at first, and then declined suddenly two months later. From 4 to 5 weeks after re-neuralization, muscle action potentials could be evoked, but the best innervation effect was found in the groups, which received re-neuralization at 2 months and 3 months after denervation. Denervation causes a progressive progress of muscle atrophy. SCs proliferate within 3 months after denervation, and then atrophy becomes irreversible from 4 months. At 4 or 5 weeks after re-neuralization, muscle action potentials can be evoked. Re-neuralization at 2 months and 3 months after denervation can achieve a good effect on the functional recovery of the atrophic muscle. Con el fin de explorar la regla de cambio de las c谷lulas precursoras miobl芍sticas (c谷lulas sat谷lite, CSs) en el m迆sculo denervado y re-inervado e investigar el mecanismo celular de los cambios morfol車gicos y funcionales del m迆sculo, se establecieron, en ratas de un mes de edad, modelos de atrofia del m迆sculo denervado y regeneraci車n del nervio. Fueron examinados de manera din芍mica 赤ndices postoperatorios tales como, el peso h迆medo del m迆sculo, 芍reas celulares de la secci車n, contenido de fibras de col芍geno y ADN, electrofisiolog赤a, n迆mero de CSs en el m迆sculo tr赤ceps de las cr赤as. Luego de la denervaci車n, el peso del m迆sculo h迆medo y el 芍rea celular se redujeron r芍pidamente, mientras que el contenido de fibras col芍genas aument車 lentamente. El n迆mero de CSs aument車 al inicio, pero m芍s tarde, a los dos meses, disminuy車 repentinamente. Entre las 4 a 5 semanas despu谷s de la reneuralizaci車n, los potenciales de acci車n muscular pudieron ser evocados, pero el mejor efecto de inervaci車n se encontr車 en los grupos que recibieron reneuralizaci車n a los 2 y 3 meses despu谷s de la denervaci車n. La denervaci車n caus車 un avance progresivo de la atrofia muscular. Las CSs proliferaron dentro de los primeros 3 meses post-denervaci車n, y luego de los 4 meses la atrofia fue irreversible
