科研之友
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摘要
Glucosidase is an indispensable enzyme in the glucose metabolism pathway of organisms. The development of sensitive and convenient detection methods for glucosidase detection is of great significance for the treatment and prevention of diabetes. In this work, a new colorimetric method for the detection of α-glucosidase activity is established using hollow CoOOH nanorings as a probe. It is found that hollow CoOOH nanorings have excellent oxidase-like activity, which can quickly catalyze dissolved oxygen to produce reactive oxide species, thus oxidizing colorless substrate 3,3′,5,5′-tetramylbenzidine (TMB) to blue product oxTMB and arising a specific absorption peak at 652 nm. However, α-glucosidase is able to hydrolyze L-ascorbate-2-glucoside and release ascorbic acid, which can transform CoOOH nanorings into Co2+ with no oxidase-like activity, thus inhibiting the formation of blue product oxTMB. Based on this mechanism, a new method for the determination of α-glucosidase activity is developed, with a detection range of 1–40 U/L and a detection limit of 0.1 U/L. This method has the advantages of low cost, simple operation, high sensitivity and good selectivity, which has been successfully applied to the detection of α-glucosidase activity in human serum.
