聚乙烯醇生物降解菌群的结构分析及优势菌株降解特性

作者:Chen Ping; Wang Chenyuan; Jia Zhirui; Jing Shuo; Zhang Yi*
来源:Chinese Journal of Environmental Engineering, 2020, 14(2): 560-568.
DOI:10.12030/j.cjee.201904151

摘要

Microbial community structure of degrading ploy (vinyl alcohol) material during the compost burial course was analyzed. The results showed that Bacillaceae was the dominant bacteria in the microbial community for ploy (vinyl alcohol) material degradation. A PVA degrading bacterial strain DG01 was isolated from PVA materials surface after three-year degradation, and was identified as Bacillus thuringiensis sp. Taking PVA concentration of and CO2 emission as indicators, the PVA degradation kinetics was explored. Results showed that PVA degradation process fitted well with the pseudo-first-order kinetic model. The correlation coefficients R2 were 0.984 0 and 0.983 5, respectively. PVA degradation conditions were optimized with single-factor experimental design under shaking flask culture. The optimum conditions for PVA biodegradation were 41℃, initial pH 7 and yeast concentration of 1.40 g?L-1. For PVA with 3 g?L-1 initial concentration, the corresponding degradation rate reached 45.21% within the first 48 h, which increased by 2.10 times. ? 2020, Science Press. All right reserved.

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