AEG1通过激活NF-κB和AP-1诱导肝癌HepG2细胞COX-2表达(英文)

作者：Huan Deng; Zhenzhen Zhou; Wei Tu; Yujia Xia; Huanjun Huang; De'an Tian

来源：The Chinese-German Journal of Clinical Oncology, 2013, 12(06): 253-256.

• hepatocellular carcinoma
• astrocyte elevated gene 1(AEG1)
• COX-2
• NF-κB
• AP-1

Objective:The aim of this study was to investigate whether astrocyte elevated gene 1 (AEG1) regulates COX-2 expression in human hepatoma HepG2 cells and related pathways involved in this process. Methods:Human hepatoma HepG2 cells were transfected with pcDNA3.1(-)-AEG1 plasmid or psilencer2.0-AEG1-shRNA1 plasmid to up/down-regulate AEG1 expression, pcDNA3.1(-) and psilencer 2.0 empty vector plasmids were transfected respectively as control. Real-time RT-PCR was carried out to measure the expression levels of AEG1 and COX-2 mRNA. The expression levels of AEG1 and COX-2 protein were detected by Western blot. NF-κB signaling was blocked by PDTC, and AP-1 signaling was blocked by curcumin. Results:AEG1 mRNA and protein levels were increased after pcDNA3.1(-)-AEG1 transfection, and decreased after psilencer2.0-AEG1-shRNAs transfection. COX-2 mRNA and protein levels were increased in AEG1-overexpressing cells and decreased in AEG1-knockdown cells. Phosphorylations of p65 and c-jun were up-regulated in AEG1-overexpr...

• 单位
  华中科技大学