高效液相色谱指纹图谱结合一测多评的山楂与野山楂有机酸比较研究

作者：Xu Shuhong; Zhou Hui; Wang Huiying; Gao Wen^*; Li Ping^*

来源：Food and Fermentation Industries, 2023, 49(6): 261-267.

DOI：10.13995/j.cnki.11-1802/ts.032157

• citric acid hydrate
• D-quinic acid
• fingerprints
• L-malic acid
• quantitative analysis of multi-components by single marker (QAMS) method

An analytical column of Agilent ZORBAX SB-Aq (4. 6 mm × 250 mm, 5 μm) was used to establish HPLC fingerprints of Crataegi Fructus (CF) and Crataegi Cuneata Fructus (CCF). With D-quinic acid as the internal reference, a quantitative analysis of multi-components by single marker (QAMS) method of D-quinic acid, L-malic acid, and citric acid hydrate was established. A total of 33 batches of CF and 10 batches of CCF were analyzed by fingerprint similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA), and partial least squares method-discriminant analysis (PLS-DA). Results showed that there were significant differences between CF and CCF in organic acids. The total amount of three organic acids in CF [(13. 02 ± 2. 85)% ] was significantly higher than that in CCF [(7. 02 ± 0. 62)% ]. Among them, the content of citric acid in CF was the highest [(8. 94 ± 2. 00)% ], followed by the L-malic acid. The content of D-quinic acid in CCF was the highest [(6. 32 ± 1. 00)% ]. The research on organic acids in CF and CCF could provide a reference for the quality evaluation and variety optimization of CF. ? 2023 Authors.

• 单位
  中国药科大学; 天然药物活性组分与药效国家重点实验室