The antihypertensive effect of compound reserpine and triamterene tablets on the regulation of intestinal flora in rat [复方利血平氨苯蝶啶片调节大鼠肠道菌群抗高血压实验研究]

作者：Jiang Y.; Liu S.; Yuan T.-Y.; Zhang W.; Li L.; Wang S.-B.; Fang L.-H.; Du G.-H.

来源：中国新药杂志, 2022, 31(11): 1057-1065.

• Aldosterone
• Compound reserpine and triamteridine tablets
• High-throughput sequencing
• Intestinal flora
• Sodium ion
• Spontaneously hypertensive rats

Objective: To observe the effect and possible mechanism of compound reserpine and triamterene tablets on the intestinal flora of spontaneously hypertensive rats (SHRs) for four weeks and explore the relationship between the therapeutic effects and regulation of intestinal flora. Methods: Forty SHR rats were randomly divided into model group (SHR), perindopril group (P), compound reserpine and triamterene tablets low-dose group (JL) and high-dose group (JH). Ten inbred Wistar rats (Wistar-Kyoto, WKY) were used as normal control group (WKY). All groups were given intragastric administration for 4 weeks. The blood pressure was measured and recorded every week. After the last administration, the samples were taken. The levels of urine sodium and potassium ions were detected. The serum was collected to detect the content of vasoactive substances aldosterone (Ald). The feces samples were collected, and total RNA of rat intestinal microbes was extracted. Subsequently, the rat gut flora was sequenced and analyzed by 16S rRNA high-throughput sequencing. Results: 4 weeks after administration, high-dose compound reserpine and triamterene tablets and perindopril significantly reduced the systolic and diastolic blood pressure in SHR rats compared with model group (P<0.05). Low-dose and high-dose compound reserpine and triamterene tablets decreased serum aldosterone levels and increased urine sodium ions, both significantly (P<0.05). Compared with the model group, low-dose and high-dose compound reserpine and triamterene tablets and perindopril increased the abundance and diversity of flora to varying degrees after intervention. The correlation analysis showed that the serum aldosterone content of SHR rats was significantly correlated with the abundance of Lactobacillaceae and unclassified p Firmicutes. The sodium ion content in urine was significantly correlated with Acidaminococcaceae and Sutterellaceae. Conclusion: Compound reserpine and triamterene tablets can improve the abundance and diversity of flora in SHR rat, and promote the growth of beneficial bacteria such as Trichospira, Prevotaceae, Muribaculaceae, Blautia and inhibit the proliferation of harmful bacteria such as Clostridium, which values in the regulation of blood pressure.

• 单位
  北京协和医学院; 中国医学科学院北京协和医院