科研之友
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摘要
Objective: To investigate the effect of Lang-chuang-ding Decoction(狼疮定汤, LCD) on the expression of DNA methylation of CD70 gene promoter in peripheral blood mononuclear cells(PBMCs) of females with systemic lupus erythematosus(SLE). Methods: PBMCs isolated from female patients with SLE or healthy donors were cultured and treated with LCD medicated serum or normal serum for 24 or 48 h. The m RNA expressions of CD70 gene in PBMCs were detected by reverse transcription polymerase chain reaction(PCR); the DNA methylation of the CD70 gene promoter region was detected by methylation-specific PCR. Results: After treated with medicated serum for 48 h, the m RNA expression levels of CD70 in PBMCs of SLE patients were significantly higher than those of healthy donors(P<0.05); the DNA methylation levels of CD70 promoter region in PBMCs of SLE patients treated with medicated serum for 48 h were significantly higher than those treated with fetal bovine serum(P<0.01). Conclusion: LCD could inhibit CD70 gene expression in PBMCs of SLE patients by promoting the DNA methylation of CD70 gene promoter.
