科研之友
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摘要
Thirty three crossbred sows (Danish Landrace ℅ Danish Large White) were fitted with jugular vein catheters through vena auricularis from one of the ears. The sows were randomly divided into three groups (Boar-, IUI- and AI-group) and blood samples were collected before, during and after service. In a final evaluation only 25 sows that became pregnant and farrowed piglets at full term were used.Cortisol concentrations increased in all groups but Boar-group (n = 8) had a significantly higher cortisol during 10 to 20 min after service than sows in AI-group (n = 8). In mated sows cortisol concentrations peaked at 15 minutes after service. The Boar-group (n = 8) showed no ascending PG-metabolite levels during the whole experiment, while both IUI- and AI-groups (n = 9 and n = 8, respectively) had a 2.5-fold increase in PG-metabolite 15 minutes after service.In conclusion, mating of sows by a boar results in a greater increase of cortisol than AI and without an elevation of PG-metabolite levels, which was seen in both the inseminated groups. It was also demonstrated that IUI-group had an earlier significant increase of PG-metabolite levels than sows inseminated conventionally. Further investigation using different semen extenders or even different type of insemination catheters might be helpful in understanding the reason for an immediate increase of PG-metabolite after insemination but not after mating.At mating, as well as during conventional artificial insemination (AI), semen is deposited into the cervical canal of the female. Semen transport along the uterine horns is elicited by myometrial contractions and a certain number of spermatozoa reach the uterotubal junction (UTJ) and the first part of isthmus. In isthmus a sperm reservoir forms in order to (i) escape reflux and phagocytosis in the uterine lumen and (ii) ensure that sufficient numbers of potentially fertile spermatozoa are present at the site of fertilization when oocytes are released [1]. Prostaglandin (PG)
