科研之友
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摘要
objective: to study the effect of perillyl alcohol on the gene expression of human pulmonary adenocarcinoma cells. methods: pulmonary adenocarcinoma cells were incubated with perillyl alcohol in dilutions ranging from 0.03% to 0.0003% for 48 hours. alterations were observed in the cell morphology, and cell viability was quantified using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays. protein synthesis of samples previously targeted with s35 was analyzed using electrophoresis on a polyacrylamide gel. expression of the proteins p53 and p44/42 was determined using the western blot method. results: after 48 hours of incubation, greater nsumbers of morphological alterations were observed in cells treated with the 0.03% perillyl alcohol dilution than in those treated with perillyl alcohol diluted to 0.003% or further. treatment with perillyl alcohol dilutions of 0.03%, 0.003% and 0.0003% inhibited cellular viability by 60.17% (p %26lt; 0.001), 15.62% (p %26lt; 0.001) and 11.53% (p %26lt; 0.05), respectively. the results show that 28-kda, 42-kda and 110-kda proteins were induced. no statistically significant effect on p53 expression was observed. in comparison with the expression of a-tubulin, the 0.003% perillyl alcohol dilution induced an increase in p42 phosphorylation and a marked decrease in p44 phosphorylation. conclusion: the results suggest that there are other, previously undescribed, metabolic pathways for perillyl alcohol effects in human pulmonary adenocarcinoma cells.
