麻疯树外植体愈伤组织中毒蛋白的Western-blot鉴定

作者:戎芳; 王胜华; 赵小光; 郭亮; 魏琴; 唐琳; 徐莺; 陈放
来源:四川大学学报(自然科学版), 2005, (01): 206-209.

摘要

The proteins from endosperm, root, stem, leaf, leafstalk of Jatropha curcas L. and their calli were hybridized to the seed toxin protein (curcin) of Jatropha curcas L. by western blot. The result showed that the curcin was specifically expressed in the endosperm and its calli, while it was not detected in root, stem, leaf, and leafstalk of Jatropha curcas L. and their calli . This study indicated that calli induced from endosperm can be used to produce curcin.