科研之友
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摘要
The present study was conducted to determine the profiles of small intestinal morphology and gene expression related to intestinal barrier function in piglets with intrauterine growth retardation. Twenty-four litters of Landrace×Large White neonatal piglets were used in this study, and one IUGR piglet and one normal birth weight (NBW) piglet were selected from each litter. Eight IUGR piglets and eight NBW piglets were selected and slaughtered at 7, 21 and 28 days of age, respectively, for small intestine sample collection and analysis. The results showed as follows: 1) compared with the NBW piglets, the villus height in jejunum of IUGR piglets at 7 days of age and the villus height in jejunum and ileum at 21 days of age were significantly decreased (P<0. 05), while the crypt depth in jejunum at 28 days of age was significantly increased (P < 0. 05). 2) Compared with the NBW piglets, the mRNA relative expression levels of zonula occluden-1 (ZO-1), claudin-1, occludin, interleukin-4 (IL-4) and interleukin-8 in jejunal mucosa of IUGR piglets at 7 days of age and the mRNA relative expression levels of ZO-1 and occludin in jejunal mucosa and ZO-1 in ileal mucosa at 21 days of age were significantly down-regulated (P < 0. 05); the mRNA relative expression levels of tumor necrosis factor-α in jejunal mucosa and interleukin-1β in ileal mucosa at 28 days of age were significantly upregulated (P<0. 05), while the mRNA relative expression level of IL-4 in ileal mucosa was significantly down-regulated (P<0. 05). In summary, in IUGR piglets, jejunum and ileum morphology is impaired, tight junction protein gene expression is down-regulated, and immune cytokines expression is abnormal, which further affects intestinal barrier function.
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