科研之友
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摘要
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate fetal hepatic karyometric and stereological alterations in the rat fetal liver resulting from the intraperitoneal administration of sodium cyclamate. The livers of ten rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas, on a precision balance; the length of the umbilical cords also were measured. After the laboratory processing, semi-seriated 6mm cuts stained with haematoxyline and eosine were performed. In seven karyometric parameters (major, minor, and medium diameters, volume, area, perimeter, and volume-area ratio), the increase was statistically significant in the treated group when compared with control group. Stereological parameters showed in the treated group a significant increase in the cytoplasmatic and cellular volume, and a significant reduction in the nucleus-cytoplasm ratio as well as in the numerical cellular density. These results showed that the sodium cyclamate in pregnant rats led to retardation of fetal development and hepatic-cellular hypertrophy in the offspring, suggesting toxicity in liver of rat fetuses El ciclamato, es una substancia derivada del 芍cido N-ciclo-hexil-sulf芍mico, bastante usada como edulcorante no cal車rica en alimentos y bebidas, as赤 como en la industria farmac谷utica. El objetivo del trabajo fue evaluar los efectos del ciclamato de sodio en h赤gados de fetos de ratas, consider芍ndose las alteraciones cariom谷tricas y estereol車gicas. Fueron utilizadas 10 ratas adultas (Rattus norvegicus) variedad Wistar, con peso medio de 240 g, siendo 5 el grupo control y 5 tratadas con ciclamato de sodio. Entre el 10 y 14 d赤a de la pre ez, 5 ratas recibieron una inyecci車n diaria intraperitoneal de 60mg/Kg/d赤a de ciclamato de sodio durante 5 d赤as. En el 20 d赤a, los animales fueron sacrificados y los fetos fijados en soluci車n de Alfac, incluidos en parafina, cortados a 6 米m y te idos com H-E. Hubo aumento estad赤sticamente significativo en siete par芍metros cariom谷tricos (di芍metros mayor, menor y medio, volumen, 芍rea, per赤metro y relaci車n 芍rea/volumen) en el grupo tratado con ciclamato de sodio comparado con el grupo
