3D Cultures of Parkinson's Disease‐Specific Dopaminergic Neurons for High Content Phenotyping and Drug Testing

作者：SilviaBolognin; MarieFossépré; XiaobingQing; JavierJarazo; Janezanar; EdinsonLucumiMoreno; SarahL Nickels; KobiWasner; NassimaOuzren; JonasWalter; AnneGrünewald; EnricoGlaab; LuisSalamanca; RonanM T Fleming; PaulM A Antony; JensC Schwamborn

来源：Advanced Science, 2019, 6(1): 1800927.

DOI：10.1002/advs.201800927

• High‐content imaging
• Microfluidics
• Parkinson's disease
• Stem cells

Abstract(#br)Parkinson's disease (PD)‐specific neurons, grown in standard 2D cultures, typically only display weak endophenotypes. The cultivation of PD patient‐specific neurons, derived from induced pluripotent stem cells carrying the LRRK2‐G2019S mutation, is optimized in 3D microfluidics. The aut...