科研之友
微信
新浪微博
Facebook
分享链接
摘要
The results confirm significantly increased plasma levels of endotoxin in bitches with pyometra and support previous reports of endotoxin involvement in the pathogenesis of the disease. Plasma concentrations of PG-metabolite were elevated in pyometra bitches and provide a good indicator of endotoxin release since the concentrations were significantly correlated to the endotoxin levels and many other hematological and chemistry parameters. The 污-globulin serum protein electrophoresis fraction and analysis of PG-metabolite can be valuable in the diagnosis of endotoxin involvement if a reliable, rapid and cost-effective test for PG-metabolite analysis becomes readily available in the future. Treatment inhibiting prostaglandin biosynthesis and related compounds could be beneficial for bitches suffering from pyometra.Pyometra (chronic purulent endometritis) is a common, metoestral disease mainly affecting middle-aged and older bitches [20,7]. Clinical symptoms are well described and derive from the site of infection (purulent vaginal discharge, abdominal pain) and more systemic effects (lethargy, depression, anorexia, polyuria, polydipsia and vomiting) [7]. Traditionally, the safest and most satisfactory treatment of pyometra is ovariohysterectomy [27]. The bacteria, predominantly isolated from the uterine content of affected bitches are Gram-negative Escherichia coli [11].Endotoxin (ET), lipopolysaccharide parts of the cell wall of Gram-negative bacteria, is released into the circulation when the bacteria grow or are destroyed. ET has many biological properties and is thought to be responsible for the systemic symptoms of pyometra in bitches [44,2,6,15]. In moderate dose, ET causes leukocytosis, fever, vomiting, depression and decrease in food consumption, whereas more severe effects are progressive hypotension leading to shock and high rates of mortality [19,31]. It has been demonstrated that ET is involved in the pathogenesis of pyometra with higher levels measured in
