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摘要
Objective: To optimize the refining technology of maggots oil and study the effect of refined maggots oil on skin aging induced by D-galactose in mice. Methods: The effects of different factors on the refining technology (including hydration degumming and deacidification by alkali-refining) of maggots oil were investigated, and the physical and chemical properties of maggots oil were analyzed. Female Kunming mice were randomly divided into normal group, model group, matrix group, vitamin E group, low dose and high dose refined maggots oil groups. Except for the normal group, the mice in the other groups were subcutaneously injected 3% D-galactose into the back of the neck for 42 consecutive days to establish the subacute aging model. The mice in each administration group were smeared with corresponding drugs once a day for 32 days. HE staining was used to observe the micromorphology of skin tissue, and the contents of water, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), hydroxyproline (HYP) and malondialdehyde (MDA) in the skin were detected. Ten rabbits were divided into normal group, intact skin group and damaged skin group. The skin irritation test was conducted on rabbits. Twelve rabbits were divided into normal group, intact skin group and damaged skin group. The rabbits were given 0.5 mL maggots essential oil on the left side of the back and olive oil of the same volume on the right side, once a day for 7 days. The irritation effect of the drug on the rabbit skin was observed, and the skin erythema and edema were scored. The skin of the administration site was stained with HE at 72 h after the last administration, and the skin tissue morphology was observed. Results: After refining, the iodine value, acid value, peroxide value and saponification value of maggots oil were (18.714±0.116) g?100 g-1, (0.465±0.006) mg?g-1, (0.016±0.001) g?100 g-1 and (130.212±1.623) mg?g-1, respectively, which conformed to the national standard of the massage essential oil and emollient oil. Compared with the model group, the dermis of mice in the maggots essential oil groups were thickened, the numbers of hair follicles and collagen fibers, the contents of water, HYP, SOD and GSH-Px in the skin increased significantly (P<0.05, P<0.01), the content of MDA decreased significantly (P<0.01). In the rabbit skin irritation test, the erythema and edema score of each group were 0, and the skin irritation was evaluated as no irritation. Conclusion: Maggots essential oil has anti-aging effect on mouse skin induced by D-galactose without irritation, and has potential application value. The color, smell, clarity and fluidity of maggots essential oil are improved after refining, which provides the basis for the development of anti-aging products of maggots essential oil.
