Deciphering the chemical profile and pharmacological mechanism of Jinlingzi powder (金铃子散) against bile reflux gastritis using ultra-high performance liquid chromatography coupled with Q Exactive Focus mass spectrometry， network pharmacology， and mole

作者：REN Hui; ZHAO Lintao; GAO Kai; YANG Yuanyuan; CUI Xiaomin; HU Jing; CHEN Zhiyong^*; LI Ye^*

来源：Journal of Traditional Chinese Medicine, 2023, 1-34.

DOI：10.19852/j.cnki.jtcm.20230908.002

• bile reflux gastritis
• liquid chromatography coupled with mass spectrometry
• network pharmacology
• molecular docking simulation
• Jinlingzi powder

OBJECTIVE: To elucidate the chemical profile and the pharmacological mechanism by which Jinlingzi powder (金铃子散， JLZP) treats bile reflux gastritis (BRG).METHODS: A BRG model was established in rats by oral administration of the model solution. JLZP was orally administered for 35 d. Residual gastric rate and tumor necrosis factor (TNF)-α， interleukin (IL)-6， and gastrin levels in the serum were measured， and stomach tissues were collected for histopathological analysis. We used ultra-high performance liquid chromatography coupled with Q Exactive Focus mass spectrometry to identify the chemical ingredients in JLZP. Then， protein-protein interaction and herb-compound-target networks were constructed to screen potential bioactive compounds and targets. Kyoto Encyclopedia of Genes and Genomes pathway analysis was then performed to elucidate the pathway involved in the JLZP-mediated treatment of BRG. After constructing the core compound-target-pathway interaction network， molecular docking was performed to study th...