科研之友
微信
新浪微博
Facebook
分享链接
摘要
Objective: To study the protective mechanism of Chinese medicine Suxiao Jiuxin Pills(速效救心丸, SXJ) on myocardial ischemia and reperfusion(I/R) injury. Methods: Mouse myocardial I/R injury model was created by 30-min coronary artery occlusion followed by 24-h reperfusion, the mice were then divided into the sham group(n=7), the I/R group(n=13), the tirofiban group(TIR, positive drug treatment, n=9), and the SXJ group(n=11). Infarct size(IS), risk region(RR), and left ventricle(LV) were analyzed with double staining methods. In addition, H9C2 rat cardiomyocytes were cultured with Na2S2O4 to simulate I/R in vitro. The phosphorylation of extracellular regulated protein kinases1/2(ERK1/2), protein kinase B(AKT), glycogen synthase kinase-3β(GSK3β), and protein expression of GATA4 in nucleus were detected with Western blot assay. Results: The ratio of IS/RR in SXJ and TIR groups were lower than that in I/R group(SXJ, 22.4%±6.6%; TIR, 20.8%±3.3%; vs. I/R, 35.4%±3.7%, P<0.05, respectively). In vitro experiments showed that SXJ increased the Na2S2O4-enhanced phosphorylation of AKT/GSK3β and nuclear expression of GATA4. Conclusion: SXJ prevents myocardial I/R injury in mice by activating AKT/GSK3β and GATA4 signaling pathways.
- 单位
