Kocuria oceani sp nov., isolated from a deep- sea hydrothermal plume

作者：Zhang, Limin; Xi, Lijun; Ruan, Jisheng; Huang, Ying^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2017, 67(1): 164-169.

DOI：10.1099/ijsem.0.001599

• GENUS
• DNA

Three strains, FXJ8.095(T), FXJ8.057 and H201, were isolated from deep-sea hydrothermal plume water collected at the Southwest Indian Ridge at a depth of 2800 m. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates formed a closely related subcluster within the genus Kocuria. The 16S rRNA gene sequence of strain FXJ8.095(T) shared 99.90 and 99.60% similarity with those of strains FXJ8.057 and H201, respectively, and 98.81, 98.75, 98.68 and 98.10% with those of `Kocuria sediminis' JCM 17929, Kocuria flava HO-9041(T), Kocuria turfanensis HO-9042(T) and Kocuria rosea JCM 11614(T), respectively. DNA-DNA hybridization values among the three new isolates were higher than 70 %, while the values between each of the isolates and the closely related type strains were well below 70 %. Random amplified polymorphic DNA fingerprint patterns and a combination of physiological and biochemical properties also distinguished the isolates from the related species. The major cellular fatty acids of the isolates were anteiso-C-15:0 and iso-C-15:0, the predominant menaquinones were MK-7(H-2) and MK-8(H-2), and the main polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C contents of strains FXJ8.095(T), FXJ8.057 and H201 were 75.6, 72.8 and 70.4 mol%, respectively. Based on the phylogenetic, phenotypic and chemotaxonomic data, we propose to classify the three strains in a novel species named Kocuria oceani sp. nov., with FXJ8.095(T) (= CGMCC 4.6946(T) = DSM 24949(T)) as the type strain.

• 单位
  中国科学院微生物研究所; 中国石油大学（北京）; 重质油国家重点实验室; 微生物资源前期开发国家重点实验室