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摘要
In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine(PE), the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by using a series of methods,including two dimensional polyacrylamide gel electrophoresis,PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS.The good two-dimensional pattern with a high resolution and reproducibility was obtained.Totally 14 differential protein spots were found. Out of these,11 proteins were preliminarily identified.Desmin,Txndc5,etc.were found to be up-regulated in PE-treated cardiomyocytes,while caspase-11, 4-hydroxyphenylpyruvic acid dioxygenase,etc.were found to be down-regulated.It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.
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单位北京大学第三医院; 中国科学院长春应用化学研究所; 长春质谱中心
