科研之友
微信
新浪微博
Facebook
分享链接
摘要
The objective of this work was identify the presence of interstitial cells of Cajal, muscle cells, nerves and androgen receptor positive cells in adult human testicle, using immunohistochemical detection for c-kit/CD-117, actin smooth muscle specific (ASMS), neurofilament (N) and androgen receptor (AR), respectively. The samples were obtained from patients (n= 10) with diagnosis of prostate c芍ncer, with surgery of orchiectomy. Subsequently were processed by histology and for immunohistochemistry using specific antibodies. It showed the presence of cells c-kit/CD-117, with diverse degrees of positivity, distributed mainly in the interstitial peritubular 芍rea of the human testicle. The peritubular myoides cells were positive to the presence of the actin smooth muscle and androgen receptor. The neurofilaments elements (+) only were observed in the vascular tunic. The specific immunohistochemistry describe the presence of the interstitial cells of Cajal in human testicular interstitium, opening a new perspective for the functional interpretation of the testicular cellularity and tubular motility. Possibly associated functionally to peribubulars cells of smooth muscle to reg迆late the mobility of the seminiferous tabules, whose integration and function would be androgen dependent. The cells that express the c-kit receptor, were found exclusively in the interstitial compartment. This cellular type in addition of the muscular cells of peritubules and the absence of nervous fibers to the interior of the testicle, could be responsible for the regulation of tubular mobility, as it happens in the gastrointestinal apparatus. El objetivo de este trabajo fue identificar la presencia de c谷lulas interticiales de Cajal, c谷lulas musculares lisas, c谷lulas nerviosas y c谷lulas que expresan receptores de andr車geno en el test赤culo de humano adulto, usando inmunohistoqu赤mica espec赤fica para: c-kit/CD-117, m迆sculo liso actina espec赤fico (ASMS), neurofilamentos (N) y para receptores de andr車genos (AR). Las muestras fueron obtenidas de pacientes (n=10) con diagn車stico de c芍ncer prost芍tico sometidos a cirug赤a de orquiectom赤a. Las biopsias se procesaron para histolog赤a e inmunohistoqu赤mica usando anticuerpos espec赤ficos. Se muestra la presencia de c谷lulas c-kit/CD-117, con diversos grados de positividad y distribuidas en el compartimento interticial del test赤culo. Las c谷lulas peritubulares mioides fueron positivas para la presencia de m迆sculo liso actina espec赤fico y para receptor de andr車genos. La marcaci車n de neurofilamentos positivos, s車lo fueron observados en la t迆nica vascular.
