Controllable Assembly of Enzymes for Multiplexed Lab‐on‐a‐Chip Bioassays with a Tunable Detection Range

作者：Dr. Yunlei Xianyu; Jing Wu; Dr. Yiping Chen; Wenshu Zheng; Prof. Mengxia Xie; Prof. Xingyu Jiang

来源：Angewandte Chemie, 2018, 130(25): 7625-7629.

DOI：10.1002/ange.201801815

• Abstimmbarer Detektionsbereich
• Chipbasierte Immunassays
• Enzymanordnung
• Klick-Chemie

Multiplexed analysis of molecules with different concentrations requires assays with a tunable detection range. A strategy is outlined that uses click chemistry to assemble horseradish peroxidase in a controlled fashion to generate enzyme assemblies as probes for multiplexed bioassays. This controllable assembly of enzymes on detection antibodies allows for lab‐on‐a‐chip immunoassays with a tunable detection range from pg mL\u003csup\u003e?1\u003c/sup\u003e to μg mL\u003csup\u003e?1\u003c/sup\u003e. Simultaneous, multiplexed bioassays of clinically relevant inflammatory biomarkers in serum are demonstrated in one lab‐on‐a‐chip format, with a limit of detection of 0.47 pg mL\u003csup\u003e?1\u003c/sup\u003e for interleukin‐6, 2.6 pg mL\u003csup\u003e?1\u003c/sup\u003e for procalcitonin, and 40 ng mL\u003csup\u003e?1\u003c/sup\u003e for C‐reactive protein. This controlled assembly technique provides a multiplexed platform for simultaneous and quantitative analyses of both low‐abundance and high‐abundance biomarkers with a broad detection range, which holds great promise as a point‐of‐care platform for biomedical diagnostics.

• 单位
  中国科学院大学