Cyanidin-3-O-β-glucoside Purified from Black Rice Protects Mice against Hepatic Fibrosis Induced by Carbon Tetrachloride via Inhibiting Hepatic Stellate Cell Activation

作者：Jiang, Xinwei; Guo, Honghui; Shen, Tianran; Tang, Xilan; Yang, Yan^*; Ling, Wenhua

来源：Journal of Agricultural and Food Chemistry, 2015, 63(27): 6221-6230.

DOI：10.1021/acs.jafc.5b02181

• cyanidin-3-O-beta-glucoside
• anthocyanins
• liver fibrosis
• hepatic stellate cells

This study investigated whether cyanidin-3-O-beta-glucoside (Cy-3-G), a predominant anthocyanin, could exert a protective role on liver injury and its further mechanisms of the anti-fibrosis actions in mice. The results demonstrated that the treatment of Cy-3-G (800 mg/kg diet) for 8 weeks significantly attenuated hepatotoxicity and fibrosis in carbon tetrachloride (CCl4) administered mice. Cy-3-G strongly down-regulated the expression of alpha-smooth muscle actin (alpha-SMA), desmin, and matrix metalloproteinase (MMPs), which showed its suppression effect on the activation of hepatic stellate cells (HSCs). In addition, Cy-3-G favorably regulated oxidative stress and apoptosis in liver. Furthermore, Cy-3-G ameliorated the infiltration of inflammatory cells such as neutrophils and leukocytes and meanwhile suppressed the production of pro-inflammatory cytokines and growth factors. In conclusion, daily intake of Cy-3-G could prevent liver fibrosis progression in mice induced by CCl4 through inhibiting HSC activation, which provides a basis for clinical practice of liver fibrosis prevention.

• 单位
  中山大学; 韶关学院