科研之友
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摘要
The restriction of the mechanisms of cell proliferation in murine seminiferous epithelium, in terms of induction of programmed cell death until recently has not been fully analyzed. The aim of this work was to assess the effect of Malathion (MP) on testicular morphology and function in mouse spermatogenesis. For the experiments, male albino mice of strain NMRI-IVIC, weighing between 30-40 g were used, and divided into control and experimental groups of 5 each. The animals of the experimental groups were injected with a single dose of MP: 241mg/kg weight (1/12 LD 50 ) resuspended in 0.9% saline, intraperitoneally. Animals were sacrificed at 8.3, 16.6 and 33.2 days post-injection (first, second and third spermatogenic cycles). Testicular samples were obtained for light microscopy (LM), transmission electron microscopy procedures, and to detect apoptosis and p53 antigen by immunohistochemical methods. Blood was collected to quantify testosterone and plasmatic cholinesterase activity. From 8.3 days, Sertoli cell vacuolization, karyolisis of pachytene spermatocytes and Leydig cells and a decreased in average of the diameter of seminiferous tubules was observed. No damage to inter-Sertoli cells junctions was detected. Percentage of seminiferous tubules showing germ cells apoptosis was increased from 8.3 days, plasmatic acetylcholinesterase activity was reduced in the group treated only 24 hours after administration of MP. Serum testosterone levels were low in treated animals at 16. 6 and 33.2 days. p53 was mostly expressed in pachytene spermatocytes from 8d. The findings of this study indicate that MP alters the testicular function affecting the DNA and interfering with spermatogenesis as well as steroidogenesis. La restricci車n de los mecanismos de proliferaci車n celular en epitelio semin赤fero murino, en t谷rminos de inducci車n de muerte celular programada hasta hace poco no hab赤a sido completamente analizada.El objetivo de este trabajo fue evaluar el efecto de malathion (MP) sobre la morfolog赤a y la funci車n testicular del rat車n.Ratones macho albinos de la cepa NMRI-IVIC, con pesos entre 30-40 g fueron utilizados, se dividieron en grupos control y experimental. Los grupos experimentales fueron inyectados por v赤a intraperitoneal con una dosis 迆nica deMP:241mg/kg de peso (1/12 DL50) resuspendido en 0,9% de soluci車n salina.Los animales fueron sacrificados en el d赤a 8,3, 16,6 y 33,2 despu谷s de la inyecci車n (primer, segundo y tercer ciclos de la espermatog谷nesis).Se obtuvieron muestras de test赤culo para estudio en microscop赤a de luz (ML), microscop赤a electr車nica de tr
