科研之友
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摘要
To strengthen the effectiveness of resveratrol (RES) as a natural antioxidant in emulsion systems, this work attempted to improve the water solubility of RES by eomplexation with SPI and to purposefully accumulate RES at the oil-water interface in emulsions by using SPI-RES complex as emulsifiers.The potential of SPI as a carrier for RES was studied through interaction studies between SPI and RES using fluorescence spectroscopy and circular dichroism (CD) spectroscopy, and the oxidative stability of emulsions stabilized by SPI-RES complex was also investigated.Upon complexation, RES showed increased water solubility.Moreover, heat treatment (90℃, 30 min) contributed to the binding of RES with SPI.The solubility of RES in water increased up to 0.82 mg/mL in 2% (w/v) heated SPI-RES complex dispersion.Fluorescence spectroscopy revealed that the SPI-RES complex is formed through hydrophobic interactions.The interaction of RES with SPI had no significant effect on protein secondary structure by using circular dichroism (CD) spectroscopy.Furthermore, SPI-RES/heated SPI-RES complex all exhibited remarkable antioxidant activities from the results of DPPH radical scavenging activity, ORAC, and reducing power.Compared to SPI/heated SPI emulsions, emulsions stabilized by SPI-RES/heated SPI-RES complex showed an enhanced oxidative stability with reduced lipid hydroperoxides, oxygen consumption rate and volatile hexanal.This improvement was believed to be mainly attributed to the targeted migration of RES to the oil-water interface during the adsorption of surfaceactive SPI, as evidenced by high interfacial accumulation of RES.
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